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Tampons from several brands that potentially millions of people use each month can contain toxic metals like lead, arsenic, and cadmium, a new study led by a UC Berkeley researcher has found.
Tampons are of particular concern as a potential source of exposure to chemicals, including metals, because the skin of the vagina has a higher potential for chemical absorption than skin elsewhere on the body. In addition, the products are used by a large percentage of the population on a monthly basis—50–80% of those who menstruate use tampons—for several hours at a time.
“Despite this large potential for public health concern, very little research has been done to measure chemicals in tampons,” said lead author Jenni A. Shearston , a postdoctoral scholar at the UC Berkeley School of Public Health and UC Berkeley’s Department of Environmental Science, Policy, & Management. “To our knowledge, this is the first paper to measure metals in tampons. Concerningly, we found concentrations of all metals we tested for, including toxic metals like arsenic and lead.”
Metals have been found to increase the risk of dementia, infertility, diabetes, and cancer. They can damage the liver, kidneys, and brain, as well as the cardiovascular, nervous, and endocrine systems. In addition, metals can harm maternal health and fetal development.
“Although toxic metals are ubiquitous and we are exposed to low levels at any given time, our study clearly shows that metals are also present in menstrual products, and that women might be at higher risk for exposure using these products,” said study co-author Kathrin Schilling , assistant professor at Columbia University Mailman School of Public Health.
Researchers evaluated levels of 16 metals (arsenic, barium, calcium, cadmium, cobalt, chromium, copper, iron, manganese, mercury, nickel, lead, selenium, strontium, vanadium, and zinc) in 30 tampons from 14 different brands. The metal concentrations varied by where the tampons were purchased (US vs. EU/UK), organic vs. non-organic, and store- vs. name-brand. However, they found that metals were present in all types of tampons; no category had consistently lower concentrations of all or most metals. Lead concentrations were higher in non-organic tampons but arsenic was higher in organic tampons.
Metals could make their way into tampons a number of ways: The cotton material could have absorbed the metals from water, air, soil, through a nearby contaminant (for example, if a cotton field was near a lead smelter), or some might be added intentionally during manufacturing as part of a pigment, whitener, antibacterial agent, or some other process in the factory producing the products.
“I really hope that manufacturers are required to test their products for metals, especially for toxic metals,” said Shearston. “It would be exciting to see the public call for this, or to ask for better labeling on tampons and other menstrual products.”
For the moment, it’s unclear if the metals detected by this study are contributing to any negative health effects. Future research will test how much of these metals can leach out of the tampons and be absorbed by the body; as well as measuring the presence of other chemicals in tampons.
Additional authors include: Kristen Upson of the College of Human Medicine, Michigan State University; Milo Gordon, Vivian Do, Olgica Balac, and Marianthi-Anna Kioumourtzoglou of Columbia University Mailman School of Public Health; and Khue Nguyen and Beizhan Yan of Lamont-Doherty Earth Observatory of Columbia University.
Funding was provided by the National Institute of Environmental Health Sciences; the National Heart, Lung, and Blood Institute; and the National Institute of Nursing Research.
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Organoids and organs-on-chips are two rapidly emerging 3D cell culture techniques that aim to bridge the gap between in vitro 2D cultures and animal models to enable clinically relevant drug discovery and model human diseases. Despite their similar goals, they use different approaches and exhibit varying requirements for implementation. Integrative approaches promise to provide improved cellular fidelity in the format of a device that can control the geometry of the organoid and provide flow, mechanical and electrical stimuli. In this Review, we discuss recent integrative approaches in the areas of intestine, kidney, lung, liver, pancreas, brain, retina, heart and tumour. We start by defining the two fields and describe how they emerged from the fields of tissue engineering, regenerative medicine and stem cells. We compare the scales at which the two methods operate and briefly describe their achievements, followed by studies integrating organoids and organ-on-a-chip devices. Finally, we define implementation limitations and requirements for translation of the integrated devices, including determining the differentiation stage at which an organoid should be placed into an organ-on-a-chip device, providing perfusable vasculature within the organoid and overcoming limitations of cell line and batch-to-batch variability.
Organoids and organs-on-chips (OoCs) aim to improve drug testing and disease modelling, but integration examples are still scarce.
The benefits of integration include organ-specific cellular hierarchy and structural fidelity; microscopic features from OoCs guiding tissue morphological formation; better reproducibility and scale-up capacities; and biocompatible built-in sensors for in situ functional readouts and industrially compatible culture formats.
A key challenge is vascularizing organoids with tissue-specific endothelial cells and aligning different cell types in organoids with appropriate flow in scalable, integrated devices.
In parallel, advances in computer vision and deep learning will be needed to enhance data processing and analysis. Addressing cell line variability and establishing validation criteria for OoC–organoid integrated devices is critical for commercial and translational success.
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Our work is funded by Canadian Institutes of Health Research (CIHR) Foundation grant FDN-167274, Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grant (RGPIN 326982-10), NSERC-CIHR Collaborative Health Research grant (CHRP 493737-16), US National Institutes of Health grant 2R01 HL076485 and a Stem Cell Network Impact Award (IMP-C4R1-3). M.R. was supported by the Killam Fellowship and Canada Research Chair. Y.Z. was supported by a CIHR postdoctoral award. S.L. was supported by a Rothschild, Zuckerman, and EMBO (ALTF 530-2022) fellowship.
These authors contributed equally: Yimu Zhao, Shira Landau.
Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
Yimu Zhao, Shira Landau, Sargol Okhovatian, Chuan Liu, Rick Xing Ze Lu, Benjamin Fook Lun Lai, Qinghua Wu, Jennifer Kieda & Milica Radisic
Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada
Yimu Zhao, Shira Landau, Sargol Okhovatian, Chuan Liu, Qinghua Wu & Milica Radisic
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada
Krisco Cheung & Milica Radisic
Department of Chemical Engineering, McMaster University, Hamilton, Ontario, Canada
Shravanthi Rajasekar & Boyang Zhang
School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada
Kimia Jozani & Boyang Zhang
Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
Milica Radisic
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Conceptualization: M.R., Y.Z., S.L. and S.O. Writing – original draft: Y.Z., S.L., S.O., C.L., R.X.Z.L., B.F.L.L., Q.W., J.K., K.C., S.R., K.J., B.Z. and M.R. Writing – review and editing: M.R., Y.Z., S.L. and S.O. Visualization: Y.Z., S.L., S.O. and K.C. Supervision: M.R. and B.Z. Project administration: M.R. Funding acquisition: M.R. and B.Z.
Correspondence to Milica Radisic .
Competing interests.
M.R., Y.Z. and B.Z. are inventors on an issued US patent for Biowire technology that is licensed to Valo Health; they receive royalties for this invention. B.Z. and S.R. are co-founders and hold equity in OrganoBiotech. The remaining authors declare no competing interests.
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Bioconvergence hub: https://bico.com/what-we-do/
Comprehensive In Vitro Pro-arrhythmia Assay (CIPA): https://cipaproject.org/
Human Cell Atlas: https://www.humancellatlas.org/
IQ consortium: https://iqconsortium.org/
United Network for Organ Sharing: https://unos.org/
Valo Health, an AI company, acquiring the heart-on-a-chip company TARA Biosystems: https://www.valohealth.com/press/valo-health-acquires-tara-biosystems-creating-first-of-its-kind-vertically-integrated-cardiovascular-platform
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Zhao, Y., Landau, S., Okhovatian, S. et al. Integrating organoids and organ-on-a-chip devices. Nat Rev Bioeng 2 , 588–608 (2024). https://doi.org/10.1038/s44222-024-00207-z
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